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lscm-equipped software  (Carl Zeiss)


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    Carl Zeiss lscm-equipped software
    Lscm Equipped Software, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lscm+software/equipped+lscm+software/pm26507362-85-5-5
    Average 90 stars, based on 1 article reviews
    lscm-equipped software - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Software:

    Article Title: Evidence for iNOS-dependent peroxynitrite production in diabetic platelets.
    Article Snippet: .. The number of platelets within the observation field were counted by Carl Zeiss LSCM software. ..

    Article Title: Hepatitis C Virus Induces CD81 and Claudin-1 Endocytosis
    Article Snippet: .. CD81 and claudin-1 localization at the plasma membrane and intracellular locations were quantified by using the overlay and intensity frequency tools included with the Zeiss LSCM software. ..

    Article Title: Effect of dacarbazine on CD44 in live melanoma cells as measured by atomic force microscopy-based nanoscopy
    Article Snippet: .. The images were processed using Zeiss LSCM software. .. Immobilization of anti-CD44 antibody on AFM cone tips with a spring constant of 0.059 N/m (DNP-10, Bruker Corp.) was as follows:17,18 1) The Si 3 N 4 tips were immersed in acetone for 5 minutes and then irradiated with ultraviolet light (mercury grille lamp, λ=253.7 nm, 250 W) for 30 minutes.

    Clinical Proteomics:

    Article Title: Hepatitis C Virus Induces CD81 and Claudin-1 Endocytosis
    Article Snippet: .. CD81 and claudin-1 localization at the plasma membrane and intracellular locations were quantified by using the overlay and intensity frequency tools included with the Zeiss LSCM software. ..

    Membrane:

    Article Title: Hepatitis C Virus Induces CD81 and Claudin-1 Endocytosis
    Article Snippet: .. CD81 and claudin-1 localization at the plasma membrane and intracellular locations were quantified by using the overlay and intensity frequency tools included with the Zeiss LSCM software. ..



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    (a) <t>Lens</t> <t>anterior</t> capsules were isolated after injury for 3, 5 and 7 days and used for whole-mount staining with LECs nuclei (blue) and EMT markers vimentin (red), Col I (green), and α-SMA (red). Images were obtained from <t>LSCM</t> 2D scanning. Scale bar = 40 μm. (b) Cryosections of injured lens at day 7 were stained with EMT markers vimentin (red), Col I (green), and α-SMA (red), and images were obtained from LSCM 2D scanning. Scale bar = 40 μm.
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    Image Search Results


    (a) Lens anterior capsules were isolated after injury for 3, 5 and 7 days and used for whole-mount staining with LECs nuclei (blue) and EMT markers vimentin (red), Col I (green), and α-SMA (red). Images were obtained from LSCM 2D scanning. Scale bar = 40 μm. (b) Cryosections of injured lens at day 7 were stained with EMT markers vimentin (red), Col I (green), and α-SMA (red), and images were obtained from LSCM 2D scanning. Scale bar = 40 μm.

    Journal: Scientific Reports

    Article Title: Quantitative analysis of injury-induced anterior subcapsular cataract in the mouse: a model of lens epithelial cells proliferation and epithelial-mesenchymal transition

    doi: 10.1038/srep08362

    Figure Lengend Snippet: (a) Lens anterior capsules were isolated after injury for 3, 5 and 7 days and used for whole-mount staining with LECs nuclei (blue) and EMT markers vimentin (red), Col I (green), and α-SMA (red). Images were obtained from LSCM 2D scanning. Scale bar = 40 μm. (b) Cryosections of injured lens at day 7 were stained with EMT markers vimentin (red), Col I (green), and α-SMA (red), and images were obtained from LSCM 2D scanning. Scale bar = 40 μm.

    Article Snippet: The confocal images containing the whole anterior subcapsular plaque were processed using the Zeiss LSCM Image Browser software (Carl Zeiss, Overkochen, Germany) for 3D stereo projections and analysis.

    Techniques: Capsules, Isolation, Staining

    The injured lens anterior capsules at day 7 were used for whole-mount staining with LECs nuclei (blue) and EMT markers vimentin (red), Col I (green), and α-SMA (red). 3D stereo images were obtained from LSCM 3D scanning. (a) Images of lens capsule 3D projections show the holistic views of the subcapsular plaques. Scale bar = 40 μm. (b) Images of lens capsule 3D projections present the depth where the base of the subcapsular plaques could reach from 90-degree rotation projection angle. Scale bar = 40 μm.

    Journal: Scientific Reports

    Article Title: Quantitative analysis of injury-induced anterior subcapsular cataract in the mouse: a model of lens epithelial cells proliferation and epithelial-mesenchymal transition

    doi: 10.1038/srep08362

    Figure Lengend Snippet: The injured lens anterior capsules at day 7 were used for whole-mount staining with LECs nuclei (blue) and EMT markers vimentin (red), Col I (green), and α-SMA (red). 3D stereo images were obtained from LSCM 3D scanning. (a) Images of lens capsule 3D projections show the holistic views of the subcapsular plaques. Scale bar = 40 μm. (b) Images of lens capsule 3D projections present the depth where the base of the subcapsular plaques could reach from 90-degree rotation projection angle. Scale bar = 40 μm.

    Article Snippet: The confocal images containing the whole anterior subcapsular plaque were processed using the Zeiss LSCM Image Browser software (Carl Zeiss, Overkochen, Germany) for 3D stereo projections and analysis.

    Techniques: Capsules, Staining

    A series of sectional images of lens anterior capsule whole-mount were acquired from LSCM 3D scanning through setting scanning interval. (a) Serial images of lens anterior capsule whole-mount stained for LECs nuclei with DAPI (blue) show extensive multilayer of cells forming plaque beneath the anterior lens capsule. Scale bar = 10 μm. (b) Serial images of α-SMA distribution (red), the hallmark of the myofibroblasts, in the subcapsular plaque of the injured lens. Scale bar = 10 μm.

    Journal: Scientific Reports

    Article Title: Quantitative analysis of injury-induced anterior subcapsular cataract in the mouse: a model of lens epithelial cells proliferation and epithelial-mesenchymal transition

    doi: 10.1038/srep08362

    Figure Lengend Snippet: A series of sectional images of lens anterior capsule whole-mount were acquired from LSCM 3D scanning through setting scanning interval. (a) Serial images of lens anterior capsule whole-mount stained for LECs nuclei with DAPI (blue) show extensive multilayer of cells forming plaque beneath the anterior lens capsule. Scale bar = 10 μm. (b) Serial images of α-SMA distribution (red), the hallmark of the myofibroblasts, in the subcapsular plaque of the injured lens. Scale bar = 10 μm.

    Article Snippet: The confocal images containing the whole anterior subcapsular plaque were processed using the Zeiss LSCM Image Browser software (Carl Zeiss, Overkochen, Germany) for 3D stereo projections and analysis.

    Techniques: Staining

    (a) Images of lens anterior capsule whole-mount stained for LECs nuclei with DAPI (blue) were acquired from LSCM 3D scanning. Using the LSCM Image Browser software, the subcapsular plaque of lens anterior capsule was manually traced (yellow outline) and the area within the tracing was shown. Screenshot of one representative lens capsule section with the subcapsular plaque area traced. The tools “Slice”, “Chan” “Overlay”, “Scale bar”, “Closed free shape curve drawing” and “Measure” which were used to quantify subcapsular plaque area are highlighted by the red circles. (b) Images of lens anterior capsule whole-mount stained for EMT marker α-SMA (red) were acquired and the area of α-SMA was manually traced (yellow outline). Screenshot of one representative lens capsule section with α-SMA area traced. The tools used to quantify α-SMA area are also highlighted by the red circles. (c) Quantification of the subcapsular plaques volumes and EMT markers distribution at day 3, 5 and 7 according to the formula of the volume of pyramid, and data were expressed as mean ± standard error of the mean (SEM) (n = 6 eyes/group).

    Journal: Scientific Reports

    Article Title: Quantitative analysis of injury-induced anterior subcapsular cataract in the mouse: a model of lens epithelial cells proliferation and epithelial-mesenchymal transition

    doi: 10.1038/srep08362

    Figure Lengend Snippet: (a) Images of lens anterior capsule whole-mount stained for LECs nuclei with DAPI (blue) were acquired from LSCM 3D scanning. Using the LSCM Image Browser software, the subcapsular plaque of lens anterior capsule was manually traced (yellow outline) and the area within the tracing was shown. Screenshot of one representative lens capsule section with the subcapsular plaque area traced. The tools “Slice”, “Chan” “Overlay”, “Scale bar”, “Closed free shape curve drawing” and “Measure” which were used to quantify subcapsular plaque area are highlighted by the red circles. (b) Images of lens anterior capsule whole-mount stained for EMT marker α-SMA (red) were acquired and the area of α-SMA was manually traced (yellow outline). Screenshot of one representative lens capsule section with α-SMA area traced. The tools used to quantify α-SMA area are also highlighted by the red circles. (c) Quantification of the subcapsular plaques volumes and EMT markers distribution at day 3, 5 and 7 according to the formula of the volume of pyramid, and data were expressed as mean ± standard error of the mean (SEM) (n = 6 eyes/group).

    Article Snippet: The confocal images containing the whole anterior subcapsular plaque were processed using the Zeiss LSCM Image Browser software (Carl Zeiss, Overkochen, Germany) for 3D stereo projections and analysis.

    Techniques: Staining, Software, Marker

    (a) The anterior capsule of mouse lens is punctured with a needle and 1 μl of 1 μM of TSA was injected into the anterior chamber of the eye immediately after injury with a microsyringe. After 7 days, lens anterior capsules were stained with LECs nuclei (blue) and EMT markers vimentin (red), Col I (green), and α-SMA (red). Images of lens capsule whole-mounts were obtained from LSCM 3D scanning. Scale bar = 40 μm. (b) Quantification of the subcapsular plaques volumes and EMT markers distribution, and data were expressed as mean ± standard error of the mean (SEM) (n = 6 eyes/group).

    Journal: Scientific Reports

    Article Title: Quantitative analysis of injury-induced anterior subcapsular cataract in the mouse: a model of lens epithelial cells proliferation and epithelial-mesenchymal transition

    doi: 10.1038/srep08362

    Figure Lengend Snippet: (a) The anterior capsule of mouse lens is punctured with a needle and 1 μl of 1 μM of TSA was injected into the anterior chamber of the eye immediately after injury with a microsyringe. After 7 days, lens anterior capsules were stained with LECs nuclei (blue) and EMT markers vimentin (red), Col I (green), and α-SMA (red). Images of lens capsule whole-mounts were obtained from LSCM 3D scanning. Scale bar = 40 μm. (b) Quantification of the subcapsular plaques volumes and EMT markers distribution, and data were expressed as mean ± standard error of the mean (SEM) (n = 6 eyes/group).

    Article Snippet: The confocal images containing the whole anterior subcapsular plaque were processed using the Zeiss LSCM Image Browser software (Carl Zeiss, Overkochen, Germany) for 3D stereo projections and analysis.

    Techniques: Injection, Capsules, Staining